The nerve cells branched out without being connected to any other tissue.

While writing a book about microscopic imaging,he came across a picture of a cell treated with Golgi’s stain.Cajal was in awe of its exquisite detail—both as a scientist and an artist.He soon set out to improve Golgi’s stain even further and create more detailed references for his artwork.By staining the tissue twice in a specific time frame,Cajal found he could stain a greater number of neurons with better resolution.And what these new slides revealed would upend reticular theory—the branches reaching out from each nerve cell were not physically connected to any other tissue.