Update on mRNA/LNP vaccine distribution, persistence, and expression

By Michael Palmer, MD
Research papers mentioned in this video:
1. Boettler, T. et al. (2022) SARS-CoV-2 vaccination can elicit a CD8 T-cell dominant hepatitis. J. Hepatol. 77:653-659
2. Fertig, T.E. et al. (2022) Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination. Biomedicines 10:1538
3. Magen, E. et al. (2022) Clinical and Molecular Characterization of a Rare Case of BNT162b2 mRNA COVID-19 Vaccine-Associated Myositis. Vaccines 10
4. Martin-Navarro, L. et al. (2022) In situ detection of vaccine mRNA in the cytoplasm of hepatocytes during COVID19 vaccine-related hepatitis. J. Hepatol. DOI:10.1016/j.jhep.2022.08.039
5.Mörz, M. (2022) A Case Report: Multifocal Necrotizing Encephalitis and Myocarditis after BNT162b2 mRNA Vaccination against Covid-19. Vaccines 10:2022060308
6. RÃķltgen, K. et al. (2022) Immune imprinting, breadth of variant recognition and germinal center response in human SARS-CoV-2 infection and vaccination. Cell DOI:10.1016/j.cell.2022.01.018
7. Bansal, S. et al. (2021) Cutting Edge: Circulating Exosomes with COVID Spike Protein Are Induced by BNT162b2 (Pfizer-BioNTech) Vaccination prior to Development of Antibodies: A Novel Mechanism for Immune Activation by mRNA Vaccines. J. Immunol. 207:2405-2410
8. Choi, S. et al. (2021) Myocarditis-induced Sudden Death after BNT162b2 mRNA COVID-19 Vaccination in Korea: Case Report Focusing on Histopathological Findings. J. Korean Med. Sci. 36:e286
9. Vuille-Lessard, E. et al. (2021) Autoimmune hepatitis triggered by SARS-CoV-2 vaccination. J. Autoimmun. 123:102710
10. Bruininks, B.M. et al. (2020) A molecular view on the escape of lipoplexed DNA from the endosome. Elife 9
11. Feng, Z. et al. (2020) Detection of the SARS-CoV-2 Nucleocaspid Protein (NP) Using Immunohistochemistry. Bio Protoc. 10
12. Francia, V. et al. (2020) The Biomolecular Corona of Lipid Nanoparticles for Gene Therapy. Bioconjug. Chem. 31:2046-2059
13. Anonymous, (2020) SARS-CoV-2 mRNA Vaccine (BNT162, PF-07302048) 2.6.4 Summary statement of the pharmacokinetic study [English translation].
14. Huang, Q. et al. (2006) Preparation of a chimeric armored RNA as a versatile calibrator for multiple virus assays. Clin. Chem. 52:1446-8