Hybrid Mushrooms w Julian Mattucci - Myc Check with Yoshi Amano ep 004

The dedikaryotization daddy himself, Julian Mattucci joins Yoshi on Myc Check to discuss fungal breeding and interspecific hybrids. Methodology, ITS sequencing, Monokaryon isolation and mating types are just a few of the topics covered. This is a must view for anybody that is trying to cross mushrooms!

0:00 introduction
1:00 methodology for making interspecific crosses
3:50 species verification is vital! / imperial scientific offers FREE ITS sequencing for active mushrooms imperialscientific.com
7:10 using the phylogenetic tree to find breeding candidates
8:40 natural v. unnatural mating / protoplasts
11:50 don't di-mon mate different species, use two monokaryons
15:00 how to confirm monokaryons
23:00 how to isolate monokaryons
25:45 Panaeolus breeding
28:00 not all sequence data in genbank is accurate!
31:00 dedikaryotization protocol / using mutants in crosses
38:35 phenohunting your new cross
39:25 separate together, parents, and offspring white board demonstration
43:40 Interspecific cross verification method
46:10 Psilocybe griseocaerulea Julian's crown jewel
50:15 ITS can sometimes match for different species / India woodlover collection
55:00 future breeding projects and goals
59:35 semperviva or subtropicalis, is it synonymous?
1:02:00 Entheome Project full genome sequences of entheogenic fungi
1:05:40 Imperial Labs is coming to Denver!
1:07:55 Julian likes Lulu and yellow umbo 🥰
1:09:50 Psychedelic research headed to Colorado! Imperial Labs Lostparadisegenetics.com
ImperialScientific.com