Covid PCR test was usually wrong

A calibration of nucleic acid (PCR) by antibody (IgG) tests in Germany: the course of SARS-CoV-2 infections estimated

https://www.frontiersin.org/journals/epidemiology/articles/10.3389/fepid.2025.1592629/full

Germany, authority-accredited laboratories (ALM)

Conducted 90% of SARS-CoV-2 polymerase chain reaction (PCR) tests

(March 2020 until January 2023)

AND

Conducted serological mass tests for IgG antibodies,

observed IgG seroprevalence trajectory.

THEREFORE

Test-positive fractions of PCR and IgG tests

Value of 0.14 found for the fitted scaling parameter

Therefore, 14% of those who were tested PCR-positively actually became infected with SARS-CoV-2.

ALSO

A quarter of the German population carried IgG antibodies from natural infections in their blood at the turn of the year from 2020 to 2021.

Second, independent analysis

Germany-specific ratio of 1:10 for ratio between one positive PCR test and the corresponding number of persons actually infected with SARS-CoV-2

Align well to perfectly with the IgG-positive fraction (92%) reported by the Robert Koch Institute at the end of 2021.

More details

Breaching the epithelial–mucosal barrier—“breach” meaning the invasion of a person’s organism by active viral material,

it is common scientific terminology to say that “the person has become infected”

Active viral material entering the mucus or epithelial cells may be bound and possibly already neutralized by IgA antibodies.

In most infection cases, particularly when symptoms occur, IgG antibodies will also become detectable in the blood.

The presence of IgG antibodies in the blood is representative of the body’s immunological memory of infections.

IgG antibodies remained detectable for up to a year in at least 90% of naturally infected SARS-CoV-2-IgG-positive individuals

PCR tests merely detect the presence of fragments of viral genetic material, not necessarily an active infection.

Relationship between PCR and IgG results is crucial, since PCR-positive counts were widely interpreted as proxies for actual infections and served as the basis for public health policy decisions.

Individuals whose PCR tests require CT values above 30 are commonly not to be considered infectious, whereas in practice, many tests were conducted with CT values up to 40

PCR assay produced positive results on water controls at cycle threshold (CT) values between 36 and 38

In short, a PCR test provides a snapshot of an individual’s current exposure to viral genetic material at the outermost layers of the body.

Lancet, 2020

https://www.thelancet.com/journals/lanres/article/PIIS2213-2600%2820%2930453-7/fulltext

RT-PCR assays in the UK have analytical sensitivity and specificity of greater than 95%

UK Government, 2020

https://www.gov.uk/government/publications/gos-impact-of-false-positives-and-negatives-3-june-2020/impact-of-false-positives-and-false-negatives-in-the-uks-covid-19-rt-pcr-testing-programme-3-june-2020

Examining data from published external quality assessments (EQAs) for RT-PCR assays for other RNA viruses carried out between 2004 to 2019

Results of 43 EQAs were examined, giving a median false positive rate of 2.3%

New England Journal of Medicine, 2024

https://www.nejm.org/doi/full/10.1056/NEJMc2313517

Among 11,297 participants who performed 76,610 days of testing, 1.7% had at least one false positive rapid antigen test.

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